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Volume 2, Issue 2, December 2018, Page: 41-46
Assessing the Incidence and Effect of Haemolysis, Lipaemia, and Icterus in Samples for Lipid and Lipoprotein Analysis
Agnes Magwete, School of Pathology, University of the Witwatersrand and NHLS, Johannesburg, South Africa
Florence Marule, National Health Laboratories, Johannesburg, South Africa
Taryn Pillay, School of Pathology, University of the Witwatersrand and NHLS, Johannesburg, South Africa
Donald M. Tanyanyiwa, School of Pathology, University of the Witwatersrand and NHLS, Johannesburg, South Africa
Received: Nov. 4, 2018;       Accepted: Nov. 26, 2018;       Published: Dec. 21, 2018
DOI: 10.11648/j.plm.20180202.13      View  337      Downloads  32
Abstract
The aims of this study were to determine the frequency, and evaluate the effects of haemolysis, icterus and lipaemia in samples for lipid lipoprotein tests in an academic medical hospital patient population. This was a retrospective study on data from the central Chemical pathology laboratory at the largest academic hospital in Africa. Serum indices (haemolysis, icterus and lipaemia) were available for all the lipid and lipoprotein chemistry specimens analysed over a 4 year (2007 – 2010) period (n=10, 5555). In the study the effects of haemolysis, lipaemia and icterus on the lipid profiles was determined. From a very large sample pool submitted to Chemical pathology laboratory for lipid and lipoprotein tests, serum indices for lipaemia, hemolysis and icterus were analysed. One thousand six hundred and eighteen (15.33%) were identified as having some form of interferent. Results were as follows: lipaemia, 13.92%; hemolysis, 1.17%; and icterus, 0.25%. There were significant differences between the clear and icteric samples in all the measured lipids and lipoproteins except HDL. Icteric samples had lower HDL levels than the clear samples. However, the frequency of icterus and haemolysis serum indices found in this study is lower than reports from other studies. The frequency of lipaemia found in this study constitutes the highest serum index at the hospital. The study concluded that lipaemia, hemolysis, and icterus occur frequently in blood specimens analysed in the laboratory. Therefore, serum indices are important interfering factors, which require an objective assessment.
Keywords
Haemolysis, Icterus, Lipaemia, Lipid and Lipoproteins and Serum Indices
To cite this article
Agnes Magwete, Florence Marule, Taryn Pillay, Donald M. Tanyanyiwa, Assessing the Incidence and Effect of Haemolysis, Lipaemia, and Icterus in Samples for Lipid and Lipoprotein Analysis, Pathology and Laboratory Medicine. Vol. 2, No. 2, 2018, pp. 41-46. doi: 10.11648/j.plm.20180202.13
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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