Volume 2, Issue 1, June 2018, Page: 5-14
Abo Blood Variants of Selected Babcock University Students and Their Link with Malaria Parasitaemia
Otajevwo Festus Dafinone, Department of Bioscience and Biotechnology, Babcock University, Ilishan Remo, Nigeria
Owodunni Olasope Mumeen, Department of Bioscience and Biotechnology, Babcock University, Ilishan Remo, Nigeria
Received: Mar. 16, 2017;       Accepted: Apr. 28, 2017;       Published: May 9, 2018
DOI: 10.11648/j.plm.20180201.12      View  662      Downloads  24
Five milliliters (5ml) of venous whole blood was collected from one hundred and eighty three students made up of 93(50.8%) male and 90(49.2%) female students of Babcock University, Ilishan Remo randomly selected across various Departments. Whole blood samples were dispensed into sequestrinized (EDTA anticoagulated) blood containers, properly mixed and labeled. Malaria Plasmodium falciparum parasite screening was done semi-quantitatively by Field stain A and B staining. ABO blood phenotyping was carried out with monoclonal Antisera A, B and D. A total of 169 (92.3%) and 14(7.7%) students were rhesus positive and negative respectively of which 92(54.4%) and 77(45.6%) samples were rhesus positive male and female students respectively and of which 1(7.1%) and 13(92.9%) students were rhesus negative male and female students respectively. One hundred and thirty five (73.8%), 36(19.7%) and 12(6.5%) of the sampled student population belonged to 17-20, 21-24 and 25-30yr age brackets respectively. One hundred and ten (60.1%), 38(20.8%), 29(15.9%) and 6(3.3%) students were of O, A, B and AB blood phenotypes respectively. Out of the 183 blood samples obtained from 93 (50.8%) and 90(49.2%) male and female students respectively, 126(68.9%) students were infected with P. falciparum malaria parasites. More males were infected than females and were significantly associated with malaria infection (X2 0.05, 1 =3.841, Cal. X2 =25.253, P˂0.05). Also, out of the 68.9% infected students, 47(37.3%) and 79(62.7%) had severe and non-severe forms of malaria infection respectively. Severe malaria frequency occurrences were 85.0%, 70.6%, 50.0% and 50.0% for blood types A, O, B and AB respectively while non-severe malaria frequency occurrences were 83.3%, 75.0%, 65.8% and 57.9% with respect to blood types A, AB O and B respectively. ABO blood types especially type A were significantly associated with severe form of P. falciparum malaria infection (X20.05, 3=7.815, X20.01, 3=11.350, Cal. X2 =284.601 and P˂0.05, P˂0.01). ABO blood types were also significantly associated with non-severe form of malaria especially type A (Cal. X2 =230.768 and hence, P˂0.05, P˂0.01). Implications of rising trend of rhesus negative factor in female students, population variations in association with ABO blood types and malaria parasitaemia are discussed.
ABO Variants, Link, Malaria Infection, Students, University
To cite this article
Otajevwo Festus Dafinone, Owodunni Olasope Mumeen, Abo Blood Variants of Selected Babcock University Students and Their Link with Malaria Parasitaemia, Pathology and Laboratory Medicine. Vol. 2, No. 1, 2018, pp. 5-14. doi: 10.11648/j.plm.20180201.12
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Garratty, G. (2000). Blood groups and disease: a historical perspective. Transfus Med Rev, 14: 291-301.
Rahman, M and Lodhi, Y. (2004). Prospects and future of conservative management of beta thalassemia major in a developing country. Pak. Journ. Med Sci. 20(2): 105-112.
Dacie, J. V. and Lewis, S. M. (2001). In: Lewis, S. M., Bain, B. J., Bates, I., Editors. (9th ed). London: Churchill Livingstone, Harcourt Publishers Limited. Practical Hematology, 444–451.
Giri, C., Ochieng, E., Tieszen, L. L and Duke, N. (2010). Status and distribution of mangrove forests of the World using earth observation satellite data. Global Ecology and Biogeography. 20(1): 154-159.
Mourant, A. E., Kopec, A C. and Domaniewska-Sobczak, K. (1976). The distribution of the human blood groups and other polymorphisms, London: Oxford University Press.
Mourant, A. E., Kopec, A. C. and Domaniewska-Sobczak, K. (1978). Blood groups and diseases: a study of associations of diseases with blood groups and other polymorphisms, London: Oxford University Press.
Talib, V. N., Aggarwall, M. P and Khuarana, S. R. (2004). Valliliv in hepatic disorders with special reference to serum hepatitis. Curr. Med. Prac. 32(8): 197-202.
Epid, T. T., Nwani, C. D. and Ugorji, N. P. (2008). Prevalence of malaria in blood donors in. Abakaliki Metropolis, Nigeria. Sci Res Essays, 3: 162-164.
Otajevwo, FD. (1997). ABO Blood group association with malaria parasitaemia among residents in Warri Delta State. Journal of Science and Technology. 4: 32-36.
Yamamoto, F. I., McNeil, P. D and Hakomori S. I. (1995). “Genomic organization of human histo-blood group ABO genes” Glycobiology. 5(1): 51-58.
Sirina, M. and Clement, O. (2013). The prevalence of malaria parasitaemia and predisposition of ABO blood groups to Plasmodium falciparum malaria among blood donors of Ghanaian Hospital. AU Journal of Technology, 16 (4); 255-266.
Ilozumba, H. C and Uzozie, C. R. (2009). Prevalence of malaria parasitaemia and its association with ABO blood group in Odoakpu Area of Onitsha South Local Government Area; Anambra State Nigeria. Nigerian Annals of Natural Science, 8(2): 1-8.
Oladeinnede, B. H; Omoregie, R; olley, M; and Anunibe, J. A. (2014). Urinary tract infection in a rural community of Nigeria. North Amer. Journ. Med. Sci. 3(2): 75-77.
Daniels, G. L., Fletcher, A., Garratty, G., Henry, S., Jorgensen, J., Judd, W. J., Levene, C., Lomas-Francis, C., Moulds, J. J., Moulds, J. M., Moulds, M., Overbeeke, M., Reid, M. E., Rouger, P., Scott, M., Sistonen, P., Smart, E., Tani, Y., Wendel, S. and Zelinski, T. (2004). Blood group terminology: from the International Society of Blood Transfusion committee on terminology for red cell surface antigens, Vox Sang, 87: 304–16.
Reid, M. E. and Lomas-Francis, C. (2004). The Blood Group Antigen Facts Book, (2nd ed). New York: Elsevier Academic Press.
Uneke, C, J., Ogbu, O. and Nwojiji, V. (2006). Potential risk of induced malaria by blood transfusion in South-eastern Nigeria. Meagul J Med.9:8-13
Cserti, C. M. and Dzik, W. H. (2007) The ABO blood group system and Plasmod Plasmodium falciparum Malaria. Blood; 110:2250-2258.
Alouch, A, L. (1997). Sickle cell disease and Malaria parasitaemia in a tropical setting Journ Biol. Chem. 12:23-30.
Agbonlahor, D. E., Obi, F. I., Esumeh, O., Ajanaku, A. A. and Igumbor, E. O. (1993). Association of ABO blood Groups and malaria parasitaemia among students of Edo State University Ekpoma, Nigeria Journal of Medical Laboratory Scientist, 4(2): 12-19.
Ruwende, C. and Hill, A. (1998). Glucose-6-phosphate dehydrogenase deficiency and malaria. Journ. Mol. Med. 76:581-588.
Sidhu, S and Sidhu, L. S. (1980). ABO blood group frequency among the sansis of Punjab. Coll. Anthropol. 4: 55-58.
Kumar, R., Mukhopadhyayi, A. K and Rao, D. N. (2010). Characterization of an N6 adenine methyl transferase from Helicobacter pylori strain which methylates adjacent adenine. FEBS Journal. 277(7): 1666-1683.
Blackwell, C. C., Dundan, S., Mackenzie, A. C., Braun, J. M and Alkrout, A. M. (2002). Blood Group and susceptibility to disease caused by Escherichia coli 0157. Journ. Infect. Dis. 185:393-396.
Athreya, B. H. and Coriell, L. L. (1967). Relation of blood groups to infection. I. A survey and review of data suggesting possible relationship between malaria and blood groups. American Journal of Epidemiology, 86: 292-304.
Singh, N., Shukla, M. M., Uniyal, V. P. and Sharma, V. P. (2003). ABO blood groups among malaria Cases from district Mandla, Madhya Pradesh. Indian J Malariol. ; 32:59-63.
Ochei and Kolhatka. (2008). Medical laboratory science theory and practice. NewDelhi: Tata Mc Graw hill publishing company limited. 233-255.
Fischer, P. R., and Boone, P. (1998). Short report: severe malaria associated with blood group. Am J Trop Med Hyg. 58:122-123.
Lell, B., May, J., Schmidt-Ott, R. J. and et al. (1999). The role of red blood cell polymorphisms in Resistance and susceptibility to malaria. Clin Infect Dis. 28:794-799.
Migot-Nabias, F, Mombo, L, E., Luty, A. J., Dubois, B., Nabias, R., Bisseye, C., Millet, P., Lu, C, Y. and Delovon, P. (2000). Human genetic factors related to susceptibility to mild malaria in Gabon. Genes Immun.1:435-44
Pathirana, S. L., Alles, H. K., Bandara, S. and et al. (2005). ABO-blood-group types and protection Against severe, Plasmodium falciparum malaria. Ann Trop Med Parasitol. ; 99:119-124.
Cserti-Gazdewich, C, M., Dhabangi, A., Musoke, C., Sewanyana, I., Mpimbaza, A. and Dzik, W. H. (2012). Cytoadherence in paediatric malaria: ABO blood groups and severe Plasmodium falciparum infection. British Journal of Haematology. 159: 223-236.
Igbineghu, C., Odaibo, G. N., Olaleye, D, O. and Odiabo, A.(2012). Malaria infection and ABO blood Grouping in Iwo community, Southwestern Nigeria. Res. Journ. Med. Sci.6:247-250.
Nasr, A. (2013). Blood group O protects against complicated Plasmodium falciparum malaria by the Mechanism of inducing high levels of antimalarial IgG antibodies. Saudi Arabia Journ. Med. 1:16-22.
Beiguelman, B., Alves, F, P., Moura, M. M., Engracia, V., Nunes, A. C. S and Heckman, M. I. O. (2003). The Association of Genetic Markers and Malaria infection in the Brazilian Western Amazonian region. Mern lnst Oswaldo Cruv. 98:455-460
Akhigbe, R. E., Ige, S. F., Afolabi, A. O., Azeez, O. M., Adegunlola, G. J. and Bamidele, J. O. (2009). Prevalence of haemoglobin variants, ABO and rhesus blood groups in Ladoke Akintola University of Technology, Ogbomoso, Nigeria. Trends Med Res, 4: 24-2.
Tursen, U., Tiftik, En., Unal, S., Gunduz, O., Kaya, T. I. and Camdevinen, H. (2005). Relationship between ABO blood groups and skin cancers. Dermatol. Journ. 11:44-47
Kassim, O. O., and Ejezie, G. C. (1982). ABO blood groups in malaria and schistosomiasis Haematobium. Acta Trop.; 39:179-184.
Opera, K. N. (2007). Onchocerciasis and ABO blood group status; a field based study. Int J Trop Med.2:123-125.
Abdulazeez, A. A., Alo, E. B. and Rebecca, S. N. (2008) Carriage rate of human immunodeficiency virus (HIV) infection among different ABO and rhesus blood groups in Adamawa State, Nigeria. Bio Med Res, 19: 41-44.
Ndambaa, Y., Gonoa, E., Nyazemab, N., Makazaa, N. and Kaonarea, K. C. (1997). Schistosomiasis Infection in relation to the ABO blood groups among school children in Zimbabwe. Acta. Trop. 65:181-190.
Herrera D, Druilhe P, Gentilini M, Eyquem A. (1982). High titres of anti-T antibodies and other haemagglutinins in human malaria. Clin Exp Immunol. 50:83- 91.
Boel, M, E., Rijken, M, J., Pimanpanarak, M., Proux, S., Nosten, F and McGready, R. (2012). No association of phenotypic ABO blood group and malaria during pregnancy. American Society of Tropical Medicine and Hygiene.8:447-45
Senga, E., Loscertales, M. P., Makwakwa, K. E., and et al. (2007). ABO blood group phenotypes Influence parity specific immunity to Plasmodium falciparum malaria in Malawian Women. Malar J.; 6:102-112.
Rowe JA, Handel IG, Thera MA. (2007). Blood group O protects against severe Plasmodium falciparum malaria through the mechanism of reduced rosetting. Proc Natl Acad Sci. USA. 104:17471-17476.
Otajevwo, F. (2013). Prevalence of malaria parasitaemia and its association with ABO blood grouping among students of Igbinedion University Okada, Nigeria. British Journal of Medicine and Medical Research. 3(4): 1164-1177.
Nkuo-Akenji, Paul, Wepngong. And Jane, F. A. (2004). Effects of ABO Rh blood groups G-6-P-D enzyme Acuity and haemoglobin genotypes on malaria parasitaemia and parasite density. Afr J Health Science. 11:93-97.
Zerihum, Abraham, Degarege. and Berhanu Erko. (2011). Association of ABO blood group and Plasmodium falciparum malaria in done Bafeno area. Southern Ethiopia. Asia Journal of Tropical cell Biomedicine.6:289-294.
Omotade, O. O., Adeyemo, A. A., Kayode, E., Falade, S, L and Ikpeme, S. (1999). Gene frequencies of ABO And RH (D) blood group alleles in a healthy infant population in Ibadan, Nigeria. West African Journal of Medicine. 18:294-297
Brooks, G, F., Butel, J, S. and Morse, S. A. (2004). Medical parasitology 23rd edition. McGraw Hill Companies. Inc. Boston; 818p
World Health Organization. Communicable diseases cluster. Trans R Soc Trop Med Hyg. 1:90-94.
Rosenfield, R. G. (1979). Blood typing and associated immunohemotological test procedures. In: manual of clinical immunology, American society for Microbiology, Washington, DC. 526:111-230
Cheesbrough, M. (2000). Parasitological tests. District laboratory practices in tropic Countries, Cambridge University Press.221:23-45.
Alemu, G and Mama, M. (2016). Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia. Malaria Research and Treatment. 10: 1-7.
Mandefro, A., Kelel, M. and Wessel, G. (2014). Association of ABO blood group and Rh factor with malaria and some gastrointestinal infectious disease in a population of Adet and Merrawi, Ethiopia. Global Journal of Biotechnology and Biochemistry. 9 (4); 137-142.
Otajevwo, F. D and Igoniwari, S. F. (2014). Malaria parasitaemia association with ABO blood types among students of a private University in western Delta, Nigeria. Inter. Jour Trop Dis. and Health. 4(5): 67-76.
Wazirzai, H., Ashfaque, A. and Herzig, J. W. (2005). Association of blood group A with increased risk coronary heart disease in the Pakistani population. Pakistan Journal of Physiology. 1(2): 1-3.
Tadesse, H. and Tadesse, K. (2013). Assessing the association of severe malaria infection and ABO blood group in northwestern Ethiopia. Journal of Vector Borne Diseases. 50(4): 292-296.
Getaneh, A and Mohammed, S. (2016). The prevalence of cardiovascular risk conditions and awareness among a Latino subgroup. Ethnicity and Disease Journ. 18(3): 342-347.
Uzoegwu, P, N. and A. E. Onuwurah. (2003). Prevalence of heamoglobinopathy and malaria diseases in the population of old Aguata Division, Anambra state, Nigeria. Biokemistri. 15:57-66
Anees, M. and M. S. Mirza. (2005). Distribution of ABO and Rh blood group alleles in Gujarat region of Punjab, Pakistan. Proc. Pak. Acad. Sci.42:233-238.
Jeremiah, Z. A. (2006). Abnormal haemoglobin variants, ABO and Rh groups among student of African Descent in Port Harcourt. Africa. Health Si.6:177-181
Bakare, A, A., M, A. Azeez and J. O. Agbolade. (2004). Gene frequencies of ABO and Rhesus blood Groups and haemoglobin variants in Ogbomosho, South-west, Nigeria global. J. Med. Sci.3:17-22
Odokuma, E, I., A, C., Okolo. And P. C. Aloamaka. (2007). Distribution of ABO and Rhesus Blood groups in Abraka. Frequency of blood groups ABO and rhesus D in the Guinea population. Nig. J. Physiol. Sci.22:89-91
Yuzhalin, A. E. and Kutikhin, A. G. (2012). ABO and blood groups in relation to ovarian, endometrial and cervical cancer risk among the population of the south-east Siberia. Asian Pacific Journal of Cancer Prevention. 13(10): 5091-5096.
Mbanugo, J, I. and Emenalo, S. E. (2004). Prevalence of malaria parasitaemia among blood donors in Owerri, imo state, Nigeria. Nigeria Journal of Parasitology. 25:75-80.
Mbanugo, J. I and Ejins, D. O. (2002) Plasmodium infections in children aged 0-5 years in Awka Metropolis, Anambra State, Nigeria. Nigerian Journal of Parasitology. 21:55-59.
Nebe, O, J., Adeoye, G, O. and Agomo, P. U. (2002). Prevalence and clinical profile of malaria among the Coastal dwellers of Lagos state, Nigeria. Nigeria Journal of Parasitology. 23:61-68.
Edington, G, M. and Gillies, H. M. (2001). Disease In: Pathology in the tropics. 121:234-245.
Ahmed, S, G., Ibrahim, U, A. and Ibrahim, G. (2001). Prevalence and clinical significance of parasitaemia On blood donors in Maiduguri, Nigeria. Nigeria Journal of Parasitology. 22:29-34.
Madhu G and Chowdhuri, A. N. R. (1980). Relationship between ABO blood groups and malaria. Bulletin of the World Health Organisation, 58 (6): 913-915.
Portilo, W. R., and Sullivan, C. C. (1979). Genetic factors that influence immune regulatory potentials in females against some diseases. Journ Biotech. 2:111-115
Beng-Ong R, Hill A. (1998). Glucose-6-phosphate dehydrogenase deficiency and malaria. J. Mol. Med. 76:581-588.
Ochei and Kolhatka. (2008). Medical laboratory science theory and practice. NewDelhi: Tata Mc Graw hill publishing company limited. 233-255.
Adam, I., Babiker, S., Mohammed, A. A., Salih, M. M., Prins, M. H and Zaki, Z. M. (2007). ABO Blood group system and placental malaria in an area of unstable malaria transmission In eastern Sudan. Malar J. 6:110-120.
Amoo AL, Newbold CI, Marsh K. (1995). Plasmodium falciparum rosetting is associated with malaria severity in Kenya. Infect Immun. 63:2323-2326.
Barragan, A., Kremsner, P. G., Wahlgren, M. and Carlson, J. (2000). Blood group A antigen is a Co receptor in Plasmodium falciparum rosetting. Infect Immun. 68:2971-2975.
Carlson J, Walgreen M. Plasmodium falciparum erythrocyte rosetting is mediated by promiscuous lectin-like interactions. J Exp Med. 1992; 176:1311-1317.
Rowe, A., Obeiro, J., Newbold, C. I. and Marsh, K. (1995). Plasmodium falciparum rosetting is Associated with malaria severity in Kenya. Infect Immun.; 63:2323-2326.
Udomsangpetch, R., Todd, J., Carlson, J., and Greenwood, B. M. (1993). The effects of Hemoglobin genotype and ABO blood group on the formation of rosettes by Plasmodium falciparum-infected red blood cells. Am J Trop Med Hyg. 48:149-153
Thakur, A., and Verma, I. C. (1992). Malaria and ABO blood groups. Indian J Malariol. 29:241- 244.
Montoya, F., Restrepo, M., Montoya, A. E. and Rojas, W. (1994). Blood groups and malaria. Rev Inst Med Trop Sao Paulo. 36:33-38.
Greene, J. O. (1993). Action Assembly perspective on social skill. Communicat. Theory. 3(1): 26-49.
Carlson J and Walgreen M. (1993). Plasmodium falciparum erythrocyte rosetting is mediated by promiscuous lectin-like interactions. J Exp Med. 176:1311-1317.
Browse journals by subject