Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase

Miraf Mekonnen; Selfu Girma; Abay Atnafu

doi:doi:10.11648/j.ijmb.20220703.14

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Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase

Miraf Mekonnen, Selfu Girma, Abay Atnafu

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 3)

Received: 9 August 2022 Accepted: 30 August 2022 Published: 14 September 2022

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Abstract

Enzymatic hydrolysis of gelatin increases its functional, textural, and nutritional characteristics. Gelatinases are enzymes used to hydrolyze gelatin into peptides and amino acids. In recent years gelatin hydrolysates have gained a considerable indication in the applications of food and non-food products. This study aimed to isolate the gelatinase enzyme from bacteria and optimize the reaction condition for gelatin hydrolysis. A total of 100 bacterial isolates were isolated from soil and water samples. All isolates were screened for the production of gelatinase on gelatin-containing media. One isolate was selected for further analysis based on the eminent properties of the enzymatic reaction. The organism can grow under solid-state fermentation and produce gelatinase enzyme with a high activity using wheat bran as a carbon source. Gelatinase was optimally active at a temperature of 50°C and at pH 8. The optimum enzyme production under solid-state fermentation includes an incubation period of 120 h and a moisture content of 66.7%. The isolate produced more enzymes with the addition of peptone as a nitrogen source. The enzyme was used to produce gelatin hydrolysate by hydrolyzing animal hooves. The production process for the gelatin hydrolysis was optimized by varying enzyme concentration and incubation time. The hydrolysis has maximum activity at 4 h incubation period and at a high amount of enzyme concentration. This study indicated that animal waste like hoofs can be converted to useful gelatin hydrolysate using microbial gelatinase and used for various applications.

Published in	International Journal of Microbiology and Biotechnology (Volume 7, Issue 3)
DOI	10.11648/j.ijmb.20220703.14
Page(s)	135-142
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Gelatinase, Hooves, Hydrolysis, Enzymes

References

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APA Style

Miraf Mekonnen, Selfu Girma, Abay Atnafu. (2022). Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase. International Journal of Microbiology and Biotechnology, 7(3), 135-142. https://doi.org/10.11648/j.ijmb.20220703.14

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ACS Style

Miraf Mekonnen; Selfu Girma; Abay Atnafu. Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase. Int. J. Microbiol. Biotechnol. 2022, 7(3), 135-142. doi: 10.11648/j.ijmb.20220703.14

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AMA Style

Miraf Mekonnen, Selfu Girma, Abay Atnafu. Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase. Int J Microbiol Biotechnol. 2022;7(3):135-142. doi: 10.11648/j.ijmb.20220703.14

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@article{10.11648/j.ijmb.20220703.14,
  author = {Miraf Mekonnen and Selfu Girma and Abay Atnafu},
  title = {Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {3},
  pages = {135-142},
  doi = {10.11648/j.ijmb.20220703.14},
  url = {https://doi.org/10.11648/j.ijmb.20220703.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220703.14},
  abstract = {Enzymatic hydrolysis of gelatin increases its functional, textural, and nutritional characteristics. Gelatinases are enzymes used to hydrolyze gelatin into peptides and amino acids. In recent years gelatin hydrolysates have gained a considerable indication in the applications of food and non-food products. This study aimed to isolate the gelatinase enzyme from bacteria and optimize the reaction condition for gelatin hydrolysis. A total of 100 bacterial isolates were isolated from soil and water samples. All isolates were screened for the production of gelatinase on gelatin-containing media. One isolate was selected for further analysis based on the eminent properties of the enzymatic reaction. The organism can grow under solid-state fermentation and produce gelatinase enzyme with a high activity using wheat bran as a carbon source. Gelatinase was optimally active at a temperature of 50°C and at pH 8. The optimum enzyme production under solid-state fermentation includes an incubation period of 120 h and a moisture content of 66.7%. The isolate produced more enzymes with the addition of peptone as a nitrogen source. The enzyme was used to produce gelatin hydrolysate by hydrolyzing animal hooves. The production process for the gelatin hydrolysis was optimized by varying enzyme concentration and incubation time. The hydrolysis has maximum activity at 4 h incubation period and at a high amount of enzyme concentration. This study indicated that animal waste like hoofs can be converted to useful gelatin hydrolysate using microbial gelatinase and used for various applications.},
 year = {2022}
}

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TY - JOUR
T1 - Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase
AU - Miraf Mekonnen
AU - Selfu Girma
AU - Abay Atnafu
Y1 - 2022/09/14
PY - 2022
N1 - https://doi.org/10.11648/j.ijmb.20220703.14
DO - 10.11648/j.ijmb.20220703.14
T2 - International Journal of Microbiology and Biotechnology
JF - International Journal of Microbiology and Biotechnology
JO - International Journal of Microbiology and Biotechnology
SP - 135
EP - 142
PB - Science Publishing Group
SN - 2578-9686
UR - https://doi.org/10.11648/j.ijmb.20220703.14
AB - Enzymatic hydrolysis of gelatin increases its functional, textural, and nutritional characteristics. Gelatinases are enzymes used to hydrolyze gelatin into peptides and amino acids. In recent years gelatin hydrolysates have gained a considerable indication in the applications of food and non-food products. This study aimed to isolate the gelatinase enzyme from bacteria and optimize the reaction condition for gelatin hydrolysis. A total of 100 bacterial isolates were isolated from soil and water samples. All isolates were screened for the production of gelatinase on gelatin-containing media. One isolate was selected for further analysis based on the eminent properties of the enzymatic reaction. The organism can grow under solid-state fermentation and produce gelatinase enzyme with a high activity using wheat bran as a carbon source. Gelatinase was optimally active at a temperature of 50°C and at pH 8. The optimum enzyme production under solid-state fermentation includes an incubation period of 120 h and a moisture content of 66.7%. The isolate produced more enzymes with the addition of peptone as a nitrogen source. The enzyme was used to produce gelatin hydrolysate by hydrolyzing animal hooves. The production process for the gelatin hydrolysis was optimized by varying enzyme concentration and incubation time. The hydrolysis has maximum activity at 4 h incubation period and at a high amount of enzyme concentration. This study indicated that animal waste like hoofs can be converted to useful gelatin hydrolysate using microbial gelatinase and used for various applications.
VL - 7
IS - 3
ER -

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Author Information

Miraf Mekonnen

Bacterial and Viral Disease Research Directorate, Armauer Hansen Research Institute, Addis Ababa, Ethiopia
Selfu Girma

Mycobacterial Disease Research Directorate, Armauer Hansen Research Institute, Addis Ababa, Ethiopia
Abay Atnafu

Pathology Unit, Armauer Hansen Research Institute, Addis Ababa, Ethiopia

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Plain Text BibTeX RIS

APA Style

Miraf Mekonnen, Selfu Girma, Abay Atnafu. (2022). Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase. International Journal of Microbiology and Biotechnology, 7(3), 135-142. https://doi.org/10.11648/j.ijmb.20220703.14

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ACS Style

Miraf Mekonnen; Selfu Girma; Abay Atnafu. Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase. Int. J. Microbiol. Biotechnol. 2022, 7(3), 135-142. doi: 10.11648/j.ijmb.20220703.14

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AMA Style

Miraf Mekonnen, Selfu Girma, Abay Atnafu. Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase. Int J Microbiol Biotechnol. 2022;7(3):135-142. doi: 10.11648/j.ijmb.20220703.14

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@article{10.11648/j.ijmb.20220703.14,
  author = {Miraf Mekonnen and Selfu Girma and Abay Atnafu},
  title = {Hydrolysis of Gelatin from Animal Hoof Using Bacterial Gelatinase},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {3},
  pages = {135-142},
  doi = {10.11648/j.ijmb.20220703.14},
  url = {https://doi.org/10.11648/j.ijmb.20220703.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220703.14},
  abstract = {Enzymatic hydrolysis of gelatin increases its functional, textural, and nutritional characteristics. Gelatinases are enzymes used to hydrolyze gelatin into peptides and amino acids. In recent years gelatin hydrolysates have gained a considerable indication in the applications of food and non-food products. This study aimed to isolate the gelatinase enzyme from bacteria and optimize the reaction condition for gelatin hydrolysis. A total of 100 bacterial isolates were isolated from soil and water samples. All isolates were screened for the production of gelatinase on gelatin-containing media. One isolate was selected for further analysis based on the eminent properties of the enzymatic reaction. The organism can grow under solid-state fermentation and produce gelatinase enzyme with a high activity using wheat bran as a carbon source. Gelatinase was optimally active at a temperature of 50°C and at pH 8. The optimum enzyme production under solid-state fermentation includes an incubation period of 120 h and a moisture content of 66.7%. The isolate produced more enzymes with the addition of peptone as a nitrogen source. The enzyme was used to produce gelatin hydrolysate by hydrolyzing animal hooves. The production process for the gelatin hydrolysis was optimized by varying enzyme concentration and incubation time. The hydrolysis has maximum activity at 4 h incubation period and at a high amount of enzyme concentration. This study indicated that animal waste like hoofs can be converted to useful gelatin hydrolysate using microbial gelatinase and used for various applications.},
 year = {2022}
}

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