The demand for both homegrown and imported frozen beef sold through super shops and online sales channels in Bangladesh has increased in recent years. These frozen beef may harbor potential microbiological health hazards which are unexplored yet. Hence, the present investigative research was envisaged to assess the potential microbiological public health hazards lying in these frozen beef. A statistically valid 72 frozen beef samples from the 204 sales points were collected during June 2021 to April 2022. The beef samples were subjected to aerobic plate count (APC) to assess the total viable bacterial load. Prevalence of major frozen meat-borne pathogens Salmonella, Escherichia coli, Campylobacter, and Listeria monocytogenes were investigated. The pathogens were isolated following US Food and Drug Administration Bacteriological Analytical Manual, henceforth, confirmatory identification was made by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technology. Real-time Polymerase Chain Reaction was used to detect pathogenic Escherichia coli. Out of 72 samples, 33 samples (46.0%) were found satisfactory, 32 samples (44.0%) were found marginal, and 7 samples (10.0%) were found unsatisfactory based on total viable bacterial load in frozen beef. Escherichia coli was identified in 63 samples (88%), but none of them was found pathogenic for humans. Based on the most probable number (MPN) of Escherichia coli, 49 samples (68%) were found satisfactory (< 50 MPN/g), 18 samples (25%) were found marginal (50 to 500 MPN/g), and 5 samples (7%) were found unsatisfactory (> 500 MPN/g). Salmonella was identified in two (2.7%), and Listeria was identified only in one (1.3%) sample. All the samples were found free from Campylobacter. Overall 93 ± 3% of frozen beef sold through different super shops in different areas in Bangladesh has been found safe for human consumption.
| Published in | International Journal of Microbiology and Biotechnology (Volume 7, Issue 2) |
| DOI | 10.11648/j.ijmb.20220702.18 |
| Page(s) | 98-105 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Beef, Quality, Salmonella, Escherichia coli, Campylobacter, Listeria
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APA Style
Md. Al-Amin, Md. Mizanur Rahman, Md. Mostofa Kamal. (2022). Microbiological Quality Assessment of Frozen Beef in Bangladesh. International Journal of Microbiology and Biotechnology, 7(2), 98-105. https://doi.org/10.11648/j.ijmb.20220702.18
ACS Style
Md. Al-Amin; Md. Mizanur Rahman; Md. Mostofa Kamal. Microbiological Quality Assessment of Frozen Beef in Bangladesh. Int. J. Microbiol. Biotechnol. 2022, 7(2), 98-105. doi: 10.11648/j.ijmb.20220702.18
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Download@article{10.11648/j.ijmb.20220702.18,
author = {Md. Al-Amin and Md. Mizanur Rahman and Md. Mostofa Kamal},
title = {Microbiological Quality Assessment of Frozen Beef in Bangladesh},
journal = {International Journal of Microbiology and Biotechnology},
volume = {7},
number = {2},
pages = {98-105},
doi = {10.11648/j.ijmb.20220702.18},
url = {https://doi.org/10.11648/j.ijmb.20220702.18},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220702.18},
abstract = {The demand for both homegrown and imported frozen beef sold through super shops and online sales channels in Bangladesh has increased in recent years. These frozen beef may harbor potential microbiological health hazards which are unexplored yet. Hence, the present investigative research was envisaged to assess the potential microbiological public health hazards lying in these frozen beef. A statistically valid 72 frozen beef samples from the 204 sales points were collected during June 2021 to April 2022. The beef samples were subjected to aerobic plate count (APC) to assess the total viable bacterial load. Prevalence of major frozen meat-borne pathogens Salmonella, Escherichia coli, Campylobacter, and Listeria monocytogenes were investigated. The pathogens were isolated following US Food and Drug Administration Bacteriological Analytical Manual, henceforth, confirmatory identification was made by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technology. Real-time Polymerase Chain Reaction was used to detect pathogenic Escherichia coli. Out of 72 samples, 33 samples (46.0%) were found satisfactory, 32 samples (44.0%) were found marginal, and 7 samples (10.0%) were found unsatisfactory based on total viable bacterial load in frozen beef. Escherichia coli was identified in 63 samples (88%), but none of them was found pathogenic for humans. Based on the most probable number (MPN) of Escherichia coli, 49 samples (68%) were found satisfactory ( 500 MPN/g). Salmonella was identified in two (2.7%), and Listeria was identified only in one (1.3%) sample. All the samples were found free from Campylobacter. Overall 93 ± 3% of frozen beef sold through different super shops in different areas in Bangladesh has been found safe for human consumption.},
year = {2022}
}
TY - JOUR T1 - Microbiological Quality Assessment of Frozen Beef in Bangladesh AU - Md. Al-Amin AU - Md. Mizanur Rahman AU - Md. Mostofa Kamal Y1 - 2022/06/30 PY - 2022 N1 - https://doi.org/10.11648/j.ijmb.20220702.18 DO - 10.11648/j.ijmb.20220702.18 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 98 EP - 105 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20220702.18 AB - The demand for both homegrown and imported frozen beef sold through super shops and online sales channels in Bangladesh has increased in recent years. These frozen beef may harbor potential microbiological health hazards which are unexplored yet. Hence, the present investigative research was envisaged to assess the potential microbiological public health hazards lying in these frozen beef. A statistically valid 72 frozen beef samples from the 204 sales points were collected during June 2021 to April 2022. The beef samples were subjected to aerobic plate count (APC) to assess the total viable bacterial load. Prevalence of major frozen meat-borne pathogens Salmonella, Escherichia coli, Campylobacter, and Listeria monocytogenes were investigated. The pathogens were isolated following US Food and Drug Administration Bacteriological Analytical Manual, henceforth, confirmatory identification was made by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technology. Real-time Polymerase Chain Reaction was used to detect pathogenic Escherichia coli. Out of 72 samples, 33 samples (46.0%) were found satisfactory, 32 samples (44.0%) were found marginal, and 7 samples (10.0%) were found unsatisfactory based on total viable bacterial load in frozen beef. Escherichia coli was identified in 63 samples (88%), but none of them was found pathogenic for humans. Based on the most probable number (MPN) of Escherichia coli, 49 samples (68%) were found satisfactory ( 500 MPN/g). Salmonella was identified in two (2.7%), and Listeria was identified only in one (1.3%) sample. All the samples were found free from Campylobacter. Overall 93 ± 3% of frozen beef sold through different super shops in different areas in Bangladesh has been found safe for human consumption. VL - 7 IS - 2 ER -
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