Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing <i>E. coli</i> Strains Isolated from Urine Samples in Njombe, Cameroon

Marthe Viviane Fonkam; Cédric Gueguim; Michel Kengne; Lucien Honoré Etame Sone; Constant Anatole Pieme

doi:doi:10.11648/j.ijmb.20220701.15

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Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon

Marthe Viviane Fonkam, Cédric Gueguim, Michel Kengne, Lucien Honoré Etame Sone, Constant Anatole Pieme

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 1)

Received: 31 December 2021 Accepted: 23 February 2022 Published: 23 March 2022

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Abstract

The emergence and spread of carbapenems resistance Enterobacteriaceae remain a major public health, a real threat as well as a silent tsunami. This phenomenon leading to reduce the therapeutic option and increase the additional cost. The general objective of this study was to determine the frequency of Escherichia coli strains producing broad-spectrum beta lactamases and resistant to carbapenems. We carried out a descriptive, cross sectional and prospective study between August and November 2020 at the Hospital Saint Jean de Malte in Njombe, on a consecutive sample of 249 patients received at the bacteriology unit. Our study population consisted of all patients who came for inpatient or outpatient consultations, were prescribed an cytobacterioligical urine exam, and in whom an E. coli strain was isolated. The identification of E. coli strains was confirmed using the Api 20E mini gallery. Resistance to carbapenems (Meropenem and Imipenem) was defined by determining the Minimal Inhibitory Concentration (MIC) by the microdilution plate method. Of the 249 cytobacterioligical urine exam samples received during our study period, 131 presented a pathogenic germ and E. coli strains were identified in 85 of them. The age of the patients in whom the E. coli strains were identified ranged from 70 to 85 years and the male sex dominated with a frequency of 38.2%. Of these identified E. coli strains, we detected 08 (9.41%) BLSE-producing strains. The resistance rate of the isolated E. coli strains producing BLSE was 75% and 50% for Meropenem and Imipenem respectively. The results of this study underline the urgent need for a regular surveillance system for broad-spectrum antibiotics in our context.

Published in	International Journal of Microbiology and Biotechnology (Volume 7, Issue 1)
DOI	10.11648/j.ijmb.20220701.15
Page(s)	37-42
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Resistance, Imipenem, Meropenem, E. coli, Broad-spectrum Beta-lactamases

References

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Marthe Viviane Fonkam, Cédric Gueguim, Michel Kengne, Lucien Honoré Etame Sone, Constant Anatole Pieme. (2022). Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon. International Journal of Microbiology and Biotechnology, 7(1), 37-42. https://doi.org/10.11648/j.ijmb.20220701.15

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Marthe Viviane Fonkam; Cédric Gueguim; Michel Kengne; Lucien Honoré Etame Sone; Constant Anatole Pieme. Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon. Int. J. Microbiol. Biotechnol. 2022, 7(1), 37-42. doi: 10.11648/j.ijmb.20220701.15

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AMA Style

Marthe Viviane Fonkam, Cédric Gueguim, Michel Kengne, Lucien Honoré Etame Sone, Constant Anatole Pieme. Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon. Int J Microbiol Biotechnol. 2022;7(1):37-42. doi: 10.11648/j.ijmb.20220701.15

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@article{10.11648/j.ijmb.20220701.15,
  author = {Marthe Viviane Fonkam and Cédric Gueguim and Michel Kengne and Lucien Honoré Etame Sone and Constant Anatole Pieme},
  title = {Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {1},
  pages = {37-42},
  doi = {10.11648/j.ijmb.20220701.15},
  url = {https://doi.org/10.11648/j.ijmb.20220701.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220701.15},
  abstract = {The emergence and spread of carbapenems resistance Enterobacteriaceae remain a major public health, a real threat as well as a silent tsunami. This phenomenon leading to reduce the therapeutic option and increase the additional cost. The general objective of this study was to determine the frequency of Escherichia coli strains producing broad-spectrum beta lactamases and resistant to carbapenems. We carried out a descriptive, cross sectional and prospective study between August and November 2020 at the Hospital Saint Jean de Malte in Njombe, on a consecutive sample of 249 patients received at the bacteriology unit. Our study population consisted of all patients who came for inpatient or outpatient consultations, were prescribed an cytobacterioligical urine exam, and in whom an E. coli strain was isolated. The identification of E. coli strains was confirmed using the Api 20E mini gallery. Resistance to carbapenems (Meropenem and Imipenem) was defined by determining the Minimal Inhibitory Concentration (MIC) by the microdilution plate method. Of the 249 cytobacterioligical urine exam samples received during our study period, 131 presented a pathogenic germ and E. coli strains were identified in 85 of them. The age of the patients in whom the E. coli strains were identified ranged from 70 to 85 years and the male sex dominated with a frequency of 38.2%. Of these identified E. coli strains, we detected 08 (9.41%) BLSE-producing strains. The resistance rate of the isolated E. coli strains producing BLSE was 75% and 50% for Meropenem and Imipenem respectively. The results of this study underline the urgent need for a regular surveillance system for broad-spectrum antibiotics in our context.},
 year = {2022}
}

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TY - JOUR
T1 - Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon
AU - Marthe Viviane Fonkam
AU - Cédric Gueguim
AU - Michel Kengne
AU - Lucien Honoré Etame Sone
AU - Constant Anatole Pieme
Y1 - 2022/03/23
PY - 2022
N1 - https://doi.org/10.11648/j.ijmb.20220701.15
DO - 10.11648/j.ijmb.20220701.15
T2 - International Journal of Microbiology and Biotechnology
JF - International Journal of Microbiology and Biotechnology
JO - International Journal of Microbiology and Biotechnology
SP - 37
EP - 42
PB - Science Publishing Group
SN - 2578-9686
UR - https://doi.org/10.11648/j.ijmb.20220701.15
AB - The emergence and spread of carbapenems resistance Enterobacteriaceae remain a major public health, a real threat as well as a silent tsunami. This phenomenon leading to reduce the therapeutic option and increase the additional cost. The general objective of this study was to determine the frequency of Escherichia coli strains producing broad-spectrum beta lactamases and resistant to carbapenems. We carried out a descriptive, cross sectional and prospective study between August and November 2020 at the Hospital Saint Jean de Malte in Njombe, on a consecutive sample of 249 patients received at the bacteriology unit. Our study population consisted of all patients who came for inpatient or outpatient consultations, were prescribed an cytobacterioligical urine exam, and in whom an E. coli strain was isolated. The identification of E. coli strains was confirmed using the Api 20E mini gallery. Resistance to carbapenems (Meropenem and Imipenem) was defined by determining the Minimal Inhibitory Concentration (MIC) by the microdilution plate method. Of the 249 cytobacterioligical urine exam samples received during our study period, 131 presented a pathogenic germ and E. coli strains were identified in 85 of them. The age of the patients in whom the E. coli strains were identified ranged from 70 to 85 years and the male sex dominated with a frequency of 38.2%. Of these identified E. coli strains, we detected 08 (9.41%) BLSE-producing strains. The resistance rate of the isolated E. coli strains producing BLSE was 75% and 50% for Meropenem and Imipenem respectively. The results of this study underline the urgent need for a regular surveillance system for broad-spectrum antibiotics in our context.
VL - 7
IS - 1
ER -

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Author Information

Marthe Viviane Fonkam

School of Health Sciences, Catholic University of Central Africa, Yaoundé, Cameroon
Cédric Gueguim

Department of Biology, Higher Institute of Medical Technology, Yaoundé, Cameroon
Michel Kengne

School of Health Sciences, Catholic University of Central Africa, Yaoundé, Cameroon
Lucien Honoré Etame Sone

Department of Biology, Higher Institute of Medical Technology, Yaoundé, Cameroon
Constant Anatole Pieme

Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon

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Marthe Viviane Fonkam, Cédric Gueguim, Michel Kengne, Lucien Honoré Etame Sone, Constant Anatole Pieme. (2022). Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon. International Journal of Microbiology and Biotechnology, 7(1), 37-42. https://doi.org/10.11648/j.ijmb.20220701.15

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Marthe Viviane Fonkam; Cédric Gueguim; Michel Kengne; Lucien Honoré Etame Sone; Constant Anatole Pieme. Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon. Int. J. Microbiol. Biotechnol. 2022, 7(1), 37-42. doi: 10.11648/j.ijmb.20220701.15

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Marthe Viviane Fonkam, Cédric Gueguim, Michel Kengne, Lucien Honoré Etame Sone, Constant Anatole Pieme. Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon. Int J Microbiol Biotechnol. 2022;7(1):37-42. doi: 10.11648/j.ijmb.20220701.15

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@article{10.11648/j.ijmb.20220701.15,
  author = {Marthe Viviane Fonkam and Cédric Gueguim and Michel Kengne and Lucien Honoré Etame Sone and Constant Anatole Pieme},
  title = {Surveillance of Resistance to Imipenem and Meropenem in Broad-spectrum Beta-lactamase-producing E. coli Strains Isolated from Urine Samples in Njombe, Cameroon},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {1},
  pages = {37-42},
  doi = {10.11648/j.ijmb.20220701.15},
  url = {https://doi.org/10.11648/j.ijmb.20220701.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220701.15},
  abstract = {The emergence and spread of carbapenems resistance Enterobacteriaceae remain a major public health, a real threat as well as a silent tsunami. This phenomenon leading to reduce the therapeutic option and increase the additional cost. The general objective of this study was to determine the frequency of Escherichia coli strains producing broad-spectrum beta lactamases and resistant to carbapenems. We carried out a descriptive, cross sectional and prospective study between August and November 2020 at the Hospital Saint Jean de Malte in Njombe, on a consecutive sample of 249 patients received at the bacteriology unit. Our study population consisted of all patients who came for inpatient or outpatient consultations, were prescribed an cytobacterioligical urine exam, and in whom an E. coli strain was isolated. The identification of E. coli strains was confirmed using the Api 20E mini gallery. Resistance to carbapenems (Meropenem and Imipenem) was defined by determining the Minimal Inhibitory Concentration (MIC) by the microdilution plate method. Of the 249 cytobacterioligical urine exam samples received during our study period, 131 presented a pathogenic germ and E. coli strains were identified in 85 of them. The age of the patients in whom the E. coli strains were identified ranged from 70 to 85 years and the male sex dominated with a frequency of 38.2%. Of these identified E. coli strains, we detected 08 (9.41%) BLSE-producing strains. The resistance rate of the isolated E. coli strains producing BLSE was 75% and 50% for Meropenem and Imipenem respectively. The results of this study underline the urgent need for a regular surveillance system for broad-spectrum antibiotics in our context.},
 year = {2022}
}

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