Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay

Khaled Ibrahim; Sabrin Aljfaeri; Ammar Aslougi; Abdlrhman Alsonosi; Mohamed Saad; Farag Ibrahim Eltaib; Mohamed-Elamen Fadel

doi:doi:10.11648/j.ijmb.20220701.12

Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay

Khaled Ibrahim, Sabrin Aljfaeri, Ammar Aslougi, Abdlrhman Alsonosi, Mohamed Saad, Farag Ibrahim Eltaib, Mohamed-Elamen Fadel

Published in International Journal of Microbiology and Biotechnology (Volume 7, Issue 1)

Received: 6 January 2022 Accepted: 28 January 2022 Published: 25 February 2022

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Abstract

Salmonella is the most common zoonotic pathogen around the world, there is an inadequate capacity of tests to detect this pathogenic bacterium in Libya. Therefore, this study was conducted to investigate the presence Salmonella spp in various foods from different food establishments in Tripoli, Libya. A total number of 370 samples were taken from 35 confectionery premises (170 samples of cakes, 25 of tarts), 11 poultry butcheries (55 samples of chicken meat), and 2 cattle butcheries (120 samples of camel meat). The isolates of Salmonella bacteria were investigated and identified by conventional cultures and biochemical methods such as (API20E). The typical Salmonella identified isolates were subjected to the PCR to detect invA gene. The results showed that 30/370 (8.11%) Salmonella spp were identified and distributed in 10 cake samples (5.9%), 2 tart samples (8%), 16 chicken meat samples (29.1%) and 2 camel meat samples (1.7%). The invA gene was detected in 22 isolates (73.33%), all Salmonella spp isolated from cakes and cattle meat samples are invasive strains. Overall, Salmonella spp is more abundant in poultry butchers than other food establishments in Tripoli, Libya, inclusion of PCR methods to detect Salmonella spp is highly recommended.

Published in	International Journal of Microbiology and Biotechnology (Volume 7, Issue 1)
DOI	10.11648/j.ijmb.20220701.12
Page(s)	11-15
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Salmonella, invA Gene, PCR, Tripoli, Libya

References

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APA Style

Khaled Ibrahim, Sabrin Aljfaeri, Ammar Aslougi, Abdlrhman Alsonosi, Mohamed Saad, et al. (2022). Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay. International Journal of Microbiology and Biotechnology, 7(1), 11-15. https://doi.org/10.11648/j.ijmb.20220701.12

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ACS Style

Khaled Ibrahim; Sabrin Aljfaeri; Ammar Aslougi; Abdlrhman Alsonosi; Mohamed Saad, et al. Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay. Int. J. Microbiol. Biotechnol. 2022, 7(1), 11-15. doi: 10.11648/j.ijmb.20220701.12

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AMA Style

Khaled Ibrahim, Sabrin Aljfaeri, Ammar Aslougi, Abdlrhman Alsonosi, Mohamed Saad, et al. Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay. Int J Microbiol Biotechnol. 2022;7(1):11-15. doi: 10.11648/j.ijmb.20220701.12

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@article{10.11648/j.ijmb.20220701.12,
  author = {Khaled Ibrahim and Sabrin Aljfaeri and Ammar Aslougi and Abdlrhman Alsonosi and Mohamed Saad and Farag Ibrahim Eltaib and Mohamed-Elamen Fadel},
  title = {Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {1},
  pages = {11-15},
  doi = {10.11648/j.ijmb.20220701.12},
  url = {https://doi.org/10.11648/j.ijmb.20220701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220701.12},
  abstract = {Salmonella is the most common zoonotic pathogen around the world, there is an inadequate capacity of tests to detect this pathogenic bacterium in Libya. Therefore, this study was conducted to investigate the presence Salmonella spp in various foods from different food establishments in Tripoli, Libya. A total number of 370 samples were taken from 35 confectionery premises (170 samples of cakes, 25 of tarts), 11 poultry butcheries (55 samples of chicken meat), and 2 cattle butcheries (120 samples of camel meat). The isolates of Salmonella bacteria were investigated and identified by conventional cultures and biochemical methods such as (API20E). The typical Salmonella identified isolates were subjected to the PCR to detect invA gene. The results showed that 30/370 (8.11%) Salmonella spp were identified and distributed in 10 cake samples (5.9%), 2 tart samples (8%), 16 chicken meat samples (29.1%) and 2 camel meat samples (1.7%). The invA gene was detected in 22 isolates (73.33%), all Salmonella spp isolated from cakes and cattle meat samples are invasive strains. Overall, Salmonella spp is more abundant in poultry butchers than other food establishments in Tripoli, Libya, inclusion of PCR methods to detect Salmonella spp is highly recommended.},
 year = {2022}
}

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TY  - JOUR
T1  - Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay
AU  - Khaled Ibrahim
AU  - Sabrin Aljfaeri
AU  - Ammar Aslougi
AU  - Abdlrhman Alsonosi
AU  - Mohamed Saad
AU  - Farag Ibrahim Eltaib
AU  - Mohamed-Elamen Fadel
Y1  - 2022/02/25
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220701.12
DO  - 10.11648/j.ijmb.20220701.12
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 11
EP  - 15
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220701.12
AB  - Salmonella is the most common zoonotic pathogen around the world, there is an inadequate capacity of tests to detect this pathogenic bacterium in Libya. Therefore, this study was conducted to investigate the presence Salmonella spp in various foods from different food establishments in Tripoli, Libya. A total number of 370 samples were taken from 35 confectionery premises (170 samples of cakes, 25 of tarts), 11 poultry butcheries (55 samples of chicken meat), and 2 cattle butcheries (120 samples of camel meat). The isolates of Salmonella bacteria were investigated and identified by conventional cultures and biochemical methods such as (API20E). The typical Salmonella identified isolates were subjected to the PCR to detect invA gene. The results showed that 30/370 (8.11%) Salmonella spp were identified and distributed in 10 cake samples (5.9%), 2 tart samples (8%), 16 chicken meat samples (29.1%) and 2 camel meat samples (1.7%). The invA gene was detected in 22 isolates (73.33%), all Salmonella spp isolated from cakes and cattle meat samples are invasive strains. Overall, Salmonella spp is more abundant in poultry butchers than other food establishments in Tripoli, Libya, inclusion of PCR methods to detect Salmonella spp is highly recommended.
VL  - 7
IS  - 1
ER  -

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Author Information

Khaled Ibrahim

Genetic Engineering Department, Biotechnology Research Center, Tripoli, Libya
Sabrin Aljfaeri

Genetic Engineering Department, Biotechnology Research Center, Tripoli, Libya
Ammar Aslougi

Genetic Engineering Department, Biotechnology Research Center, Tripoli, Libya
Abdlrhman Alsonosi

Medical Microbiology Department, Faculty of Medicine, University of Sebha, Sebha, Libya
Mohamed Saad

Genetic Engineering Department, Biotechnology Research Center, Tripoli, Libya
Farag Ibrahim Eltaib

Genetic Engineering Department, Biotechnology Research Center, Tripoli, Libya
Mohamed-Elamen Fadel

Medical Laboratory Department, University of Sebha, Sabha, Libya

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APA Style

Khaled Ibrahim, Sabrin Aljfaeri, Ammar Aslougi, Abdlrhman Alsonosi, Mohamed Saad, et al. (2022). Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay. International Journal of Microbiology and Biotechnology, 7(1), 11-15. https://doi.org/10.11648/j.ijmb.20220701.12

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ACS Style

Khaled Ibrahim; Sabrin Aljfaeri; Ammar Aslougi; Abdlrhman Alsonosi; Mohamed Saad, et al. Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay. Int. J. Microbiol. Biotechnol. 2022, 7(1), 11-15. doi: 10.11648/j.ijmb.20220701.12

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AMA Style

Khaled Ibrahim, Sabrin Aljfaeri, Ammar Aslougi, Abdlrhman Alsonosi, Mohamed Saad, et al. Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay. Int J Microbiol Biotechnol. 2022;7(1):11-15. doi: 10.11648/j.ijmb.20220701.12

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@article{10.11648/j.ijmb.20220701.12,
  author = {Khaled Ibrahim and Sabrin Aljfaeri and Ammar Aslougi and Abdlrhman Alsonosi and Mohamed Saad and Farag Ibrahim Eltaib and Mohamed-Elamen Fadel},
  title = {Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {7},
  number = {1},
  pages = {11-15},
  doi = {10.11648/j.ijmb.20220701.12},
  url = {https://doi.org/10.11648/j.ijmb.20220701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20220701.12},
  abstract = {Salmonella is the most common zoonotic pathogen around the world, there is an inadequate capacity of tests to detect this pathogenic bacterium in Libya. Therefore, this study was conducted to investigate the presence Salmonella spp in various foods from different food establishments in Tripoli, Libya. A total number of 370 samples were taken from 35 confectionery premises (170 samples of cakes, 25 of tarts), 11 poultry butcheries (55 samples of chicken meat), and 2 cattle butcheries (120 samples of camel meat). The isolates of Salmonella bacteria were investigated and identified by conventional cultures and biochemical methods such as (API20E). The typical Salmonella identified isolates were subjected to the PCR to detect invA gene. The results showed that 30/370 (8.11%) Salmonella spp were identified and distributed in 10 cake samples (5.9%), 2 tart samples (8%), 16 chicken meat samples (29.1%) and 2 camel meat samples (1.7%). The invA gene was detected in 22 isolates (73.33%), all Salmonella spp isolated from cakes and cattle meat samples are invasive strains. Overall, Salmonella spp is more abundant in poultry butchers than other food establishments in Tripoli, Libya, inclusion of PCR methods to detect Salmonella spp is highly recommended.},
 year = {2022}
}

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TY  - JOUR
T1  - Study on the Incidence of Salmonella Species in Different Food Samples by Using Cultural and Rapid invA Gene Specific PCR-Based Assay
AU  - Khaled Ibrahim
AU  - Sabrin Aljfaeri
AU  - Ammar Aslougi
AU  - Abdlrhman Alsonosi
AU  - Mohamed Saad
AU  - Farag Ibrahim Eltaib
AU  - Mohamed-Elamen Fadel
Y1  - 2022/02/25
PY  - 2022
N1  - https://doi.org/10.11648/j.ijmb.20220701.12
DO  - 10.11648/j.ijmb.20220701.12
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 11
EP  - 15
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20220701.12
AB  - Salmonella is the most common zoonotic pathogen around the world, there is an inadequate capacity of tests to detect this pathogenic bacterium in Libya. Therefore, this study was conducted to investigate the presence Salmonella spp in various foods from different food establishments in Tripoli, Libya. A total number of 370 samples were taken from 35 confectionery premises (170 samples of cakes, 25 of tarts), 11 poultry butcheries (55 samples of chicken meat), and 2 cattle butcheries (120 samples of camel meat). The isolates of Salmonella bacteria were investigated and identified by conventional cultures and biochemical methods such as (API20E). The typical Salmonella identified isolates were subjected to the PCR to detect invA gene. The results showed that 30/370 (8.11%) Salmonella spp were identified and distributed in 10 cake samples (5.9%), 2 tart samples (8%), 16 chicken meat samples (29.1%) and 2 camel meat samples (1.7%). The invA gene was detected in 22 isolates (73.33%), all Salmonella spp isolated from cakes and cattle meat samples are invasive strains. Overall, Salmonella spp is more abundant in poultry butchers than other food establishments in Tripoli, Libya, inclusion of PCR methods to detect Salmonella spp is highly recommended.
VL  - 7
IS  - 1
ER  -

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