@article{10.11648/j.ijmb.20210603.14,
  author = {Nguimbi Etienne and Soloka Mabika Faly Armel and Dibangou Valentin and Nzaou Stech Anomène Eckzechel and Kayath Aimé Christian and Moyen Rachel},
  title = {New Phylogenetic Molecular Markers in Bacteria of the Genus Bacillus: Fibrinolytic Proteases},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {6},
  number = {3},
  pages = {86-94},
  doi = {10.11648/j.ijmb.20210603.14},
  url = {https://doi.org/10.11648/j.ijmb.20210603.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210603.14},
  abstract = {The fibrinolytic proteases developed by bacteria have become substances of medical interest, as they have been recognized as antithrombotic substances in the blood. It is in this context that a study has been carried out with the aim of studying the fibrinolytic proteases produced by bacteria isolated from food in the Congo. Four strains of bacteria of the genus Bacillus isolated from crushed and cooked squash seeds, namely Bacillus sp strain ASM7, Bacillus sp strain CRK, Bacillus pumilus strain ASM5, Bacillus subtilis strain ASM1, identified by their rDNA16S, were tested positive for the production of fibrinolytic proteases. The fibrin box technique was used. The diameters on the fibrin boxes prove a significant production of fibrinolytic proteases. The genes coding for these fibrinolytic proteases were amplified by PCR and 1% Agarose gel electrophoresis shows that the size of the amplicons for the four strains is between1200-1450bp. The sequences of these coding genes have been for the four strains studied submitted to GenBank and the assigned accession numbers are respectively: Bacillus sp strain ASM7 MT743004, Bacillus sp strain CRK MT743005, Bacillus pumilus strain ASM5 MT743006, Bacillus subtilis strain ASM1 MT743007. These genes show a high degree of similarity of almost 99.50% with their counterparts in the databases, are all coding and show some observable differences. The translation of these genes in coherent reading frames confirms the amino acids already known in the active sites relating to their fibrinolysis role. The fibrinolytic protease CFE1 (Id=QNJ60181) is for Bacillus sp strain ASM7, the fibrinolytic protease CFE2 (Id=QNJ60182) is for Bacillus sp strain CRK, the fibrinolytic protease CFE3 (Id=QNJ60183) is for Bacillus pumilus strain ASM5, the fibrinolytic protease CFE4 (Id=QNJ60184) is for Bacillus subtilis strain ASM1. All these fibrinolytic proteases show a strong similarity (99.51-99.76%) with the Bacillus AprX serine protease, reference sequence. The phylogenetic inference test based on these fibrinolytic proteases shows that these proteases form a highly conserved characteristic group in bacteria of the genus Bacillus. This allowed us to retain these fibrinolytic proteases as an important phylogenetic molecular marker.},
 year = {2021}
}

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TY  - JOUR
T1  - New Phylogenetic Molecular Markers in Bacteria of the Genus Bacillus: Fibrinolytic Proteases
AU  - Nguimbi Etienne
AU  - Soloka Mabika Faly Armel
AU  - Dibangou Valentin
AU  - Nzaou Stech Anomène Eckzechel
AU  - Kayath Aimé Christian
AU  - Moyen Rachel
Y1  - 2021/08/18
PY  - 2021
N1  - https://doi.org/10.11648/j.ijmb.20210603.14
DO  - 10.11648/j.ijmb.20210603.14
T2  - International Journal of Microbiology and Biotechnology
JF  - International Journal of Microbiology and Biotechnology
JO  - International Journal of Microbiology and Biotechnology
SP  - 86
EP  - 94
PB  - Science Publishing Group
SN  - 2578-9686
UR  - https://doi.org/10.11648/j.ijmb.20210603.14
AB  - The fibrinolytic proteases developed by bacteria have become substances of medical interest, as they have been recognized as antithrombotic substances in the blood. It is in this context that a study has been carried out with the aim of studying the fibrinolytic proteases produced by bacteria isolated from food in the Congo. Four strains of bacteria of the genus Bacillus isolated from crushed and cooked squash seeds, namely Bacillus sp strain ASM7, Bacillus sp strain CRK, Bacillus pumilus strain ASM5, Bacillus subtilis strain ASM1, identified by their rDNA16S, were tested positive for the production of fibrinolytic proteases. The fibrin box technique was used. The diameters on the fibrin boxes prove a significant production of fibrinolytic proteases. The genes coding for these fibrinolytic proteases were amplified by PCR and 1% Agarose gel electrophoresis shows that the size of the amplicons for the four strains is between1200-1450bp. The sequences of these coding genes have been for the four strains studied submitted to GenBank and the assigned accession numbers are respectively: Bacillus sp strain ASM7 MT743004, Bacillus sp strain CRK MT743005, Bacillus pumilus strain ASM5 MT743006, Bacillus subtilis strain ASM1 MT743007. These genes show a high degree of similarity of almost 99.50% with their counterparts in the databases, are all coding and show some observable differences. The translation of these genes in coherent reading frames confirms the amino acids already known in the active sites relating to their fibrinolysis role. The fibrinolytic protease CFE1 (Id=QNJ60181) is for Bacillus sp strain ASM7, the fibrinolytic protease CFE2 (Id=QNJ60182) is for Bacillus sp strain CRK, the fibrinolytic protease CFE3 (Id=QNJ60183) is for Bacillus pumilus strain ASM5, the fibrinolytic protease CFE4 (Id=QNJ60184) is for Bacillus subtilis strain ASM1. All these fibrinolytic proteases show a strong similarity (99.51-99.76%) with the Bacillus AprX serine protease, reference sequence. The phylogenetic inference test based on these fibrinolytic proteases shows that these proteases form a highly conserved characteristic group in bacteria of the genus Bacillus. This allowed us to retain these fibrinolytic proteases as an important phylogenetic molecular marker.
VL  - 6
IS  - 3
ER  - 

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